Study of Bull Spermatozoa

Effect of the Addition of Insulin Like Growth Factor-I (IGF-I) on Cryopreservation of Hariana Bull Semen

This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...

O-12: Studies on Sequestration of PDC-109 Protein on Cryodamage and In Vitro Fertility of Crossbred Bull Spermatozoa

Background: Plasma membrane of the spermatozoa interacts with and is altered by abundant seminal PDC-109 present in their immediate milieu in vivo with damaging effect in a time- and concentration- dependent manner. Therefore we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial for cryopreservation of sperm cells. To this aim we evaluated the effect of s...

Spermatozoa Molecules in Relation to Bulls Fertility

Bull fertility may be defined as the process by which spermatozoa fertilize and activate the ovum and then support embryonic development. Bull fertility is a complex trait having relatively low heritability and plays a vital role for efficient production and reproduction of bovine. Various mechanisms involved in regulating bull fertility associated phenotype and reliable biomarkers are poorly d...

RNA extraction and leptin receptor mRNA detection in bull ejaculated spermatozoa

Anti-oxidative Effects of Ethanol Extract of Origanum vulgare on Kinetics, Microscopic and Oxidative Parameters of Cryopreserved Holstein Bull Spermatozoa

Origanum vulgare contains high levels of phenolic compounds such as gallic acid and polyphenols such as rosmarinic acid and quercetin. The purpose of this study was to investigate the effects of Origanum vulgare extract, as a natural antioxidant, on freezing-thawing semen quality in Holstein bulls. Three Holstein bulls (5-6 years old, mean live weight 800 kg) were used for semen collection twic...

The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa

The aim of this study was to determine the effects of the antioxidants superoxide dismutase (SOD),glutathione peroxidase (GPx) and butylated hydroxytoluene (BHT) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. Ejaculates were collected from five Holsteinbulls and pooled at 37°C. The semen samples were diluted with a CEY extender containing addit...